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Module Number:
| 12776
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| Module Title: | Eukaryotic Microorganisms/Microalgae |
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Eukaryotische Mikroorganismen/Mikroalgen
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| Department: |
Faculty 2 - Environment and Natural Sciences
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| Responsible Staff Member: | -
Prof. Dr. rer. nat. Scheibner, Katrin
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| Language of Teaching / Examination: | English |
| Duration: | 1 semester |
| Frequency of Offer: |
Every summer semester
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| Credits: |
8
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| Learning Outcome: | In the laboratory course the practical skills of the students by applying different methods for the classification and cultivation of eukaryotic microorganisms (e.g. heterotrophic marine dinoflagellates, algae-like peronosporomycetes, lower and higher fungi), for the development of culture conditions, the quantitative and qualitative analysis of cell growth, the extraction of natural substances (e.g. fatty acids, enzymes) and the analytical product identification with chromatographic methods are trained. Herein each student should isolate and determine eukaryotic microorganisms from natural sources, purify one strain and determine contents and activities by using screening techniques.
The students work in small teams (2-3 persons) and coordinate the approaches/practical work between the different group members. In this way, the students improve their soft skills in terms of communication and coordination of the work flow. Additionally, the laboratory course will strengthen the students competencies in the interpretation, evaluation and presentation of lab data by summarizing the results in a final report and by an oral presentation at the end of the course. The accompanying seminar intents to train the scientific competencies of the students by searching for and presenting relevant literature focusing on the topics of the practical course. |
| Contents: | Contents of the Lab Course:- preparation of labware and cultivation media
- inoculation of eukaryotic microorganism cultures using culture flask/bioreactors
- monitoring of culture parameters (pH, OD, biomass dry weight, enzyme activities)
- sampling at different time points during the cultivation
- sample preparation: cell disintegration (e.g. french press), hydrolysis, dialysis, micro-/ultrafiltration, freeze-drying
- liquid extraction of lipophilic compounds (e.g. PUFA´s)
- photometric assays for the determination of protein content and enzyme activity
- protein purification using FPLC
- compound analysis with HPLC/ELSD/DAD and LCMS
- data evaluation, presentation, report
Contents of the Seminar:
- The seminar course will give a current overview to the state of the art of science in those topics and microorganisms which are in the focus of the practical course.
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| Recommended Prerequisites: | Techniques in microbiological isolation and cultivation; protein purification; analytical photometric and chromatographic methods. |
| Mandatory Prerequisites: | Only applies to the Master Biotechnology PO 2018 with 4 semesters standard period of study:
Successfull completion of module
- 12739 - Research Internship
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| Forms of Teaching and Proportion: | -
Laboratory training
/ 7 Hours per Week per Semester
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Seminar
/ 1 Hours per Week per Semester
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Self organised studies
/ 120 Hours
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| Teaching Materials and Literature: | - Madigan M. T., Martinko J. M., Bender K.: Brock Biology of Microorganisms. Pearson 2018, ISBN 9781292235103
- Esser K., Hofrichter M.: The Mycota - A Comprehensive Treatise on Fungi as Experimental Systems for Basic and Applied Research, Industrial Application X. Springer 2010, ISBN 9783642114571
- Dörfelt H., Jetschke G.: Wörterbuch der Mycologie. Spektrum 2001, ISBN 9783827409201
- Bucholz K., Kasche V., Bornscheuer U. T.: Biocatalysts and Enzyme Technology. Wiley-Blackwell, 2012, ISBN: 9783527329892
Several publications e.g.:
- Yue Jiang, Feng Chen, Shi-Zhong Liang (1996). Production potential of docosahexaenoic acid by the heterotrophic marine dinoflagellate Crypthecodinium cohnii. Process Biochemistry 34: 633–637
- Blackman, L.M., Cullerne, D.P. & Hardham, A.R. (2014) Bioinformatic characterisation of genes encoding cell wall degrading enzymes in the Phytophthora parasitica genome. BMC Genomics 15, 785 (2014). https://doi.org/10.1186/1471-2164-15-785
- Olmedo, A., Aranda, C., del Rio, J. C., Kiebist, J., Scheibner, K., Martínez, A. T. & Gutiérrez, A. (2016). From Alkanes to Carboxylic Acids: Terminal Oxygenation by a Fungal Peroxygenase. Angewandte Chemie International Edition, Vol. 55(40): 12248-51 (doi:10.1002/anie.201605430)
- C. André Lévesque (2011). Fifty years of oomycetes—from consolidation to evolutionary and genomic exploration. Fungal Diversity 50:35–46 DOI 10.1007/s13225-011-0128-7
- Friedrich, S., Gröbe, G., Kluge, M., Brinkmann, T., Hofrichter, M. & Scheibner, K. (2014). Optimization of a biocatalytic process to gain (R)-1-phenylethanol by applying the software tool Sabento for ecological assessment during the early stages of development. Journal of Molecular Catalysis B: Enzymatic, Vol. 103: 36-40. (doi:10.1016/j.molcatb.2013.10.002)
- Kiebist, J., Schmidtke, K.-U., Zimmermann, J., Kellner, H., Jemlich, N., Ulrich, R., Zänder, D., Hofrichter, M. & Scheibner, K. (2017). A peroxygenase from Chaetomium globosum catalyzes the selective oxygenation of testosterone. ChemBioChem, Vol. 18: 563-569 (doi: 10.1002/cbic.201600677)
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| Module Examination: | Continuous Assessment (MCA) |
| Assessment Mode for Module Examination: | - Lab work-preparation of experiment, performance, discussion, time management (50%)
- Presentation and discussion in the seminar, duration depends on the topic (20%)
- Oral Test/ final colloquium, duration: 15 min (20%)
- Report, c. 20 pages (10%)
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| Evaluation of Module Examination: | Performance Verification – graded |
| Limited Number of Participants: | 8 |
| Part of the Study Programme: | -
Master (applied) /
Biotechnology /
PO 2018
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Master (applied) - Extended Semester /
Biotechnology /
PO 2018
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Abschluss im Ausland /
Environmental and Resource Management /
keine PO
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| Remarks: | The module will be made up for once in the winter semester 2021/22. |
| Module Components: | - Lab Course "Eukaryotic Microorganisms"
- Seminar "Eukaryotic Microorganisms"
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| Components to be offered in the Current Semester: | |
