12798 - Methods in Bioanalytics Modulübersicht

Module Number: 12798
Module Title:Methods in Bioanalytics
  Methoden in der Bioanalytik
Department: Faculty 2 - Environment and Natural Sciences
Responsible Staff Member:
  • Dr. rer. nat. habil. Rödiger, Stefan
Language of Teaching / Examination:English
Duration:1 semester
Frequency of Offer: Every summer semester
Credits: 8
Learning Outcome:First the students expand their professional competencies in training the skills in sterile cell culture techniques and practice the performance of special cell assays (incl. analysis of DNA damage upon treatment with single (etoposide) and multiple chemotherapeutic substances, FISH to asses genomic alterations of cancer cells). In particular, the cell assays have relevance in precision medicine and pharmacology/pharmaceutical research. Furthermore, they broaden their methodological background in analysis, interpretation and reporting of lab data by different methods used in applied statistical bioinformatics, forensics, pharmaceutical research and precision medicine. By working in small groups the students train also soft skills like the ability to work and communicate in a team.
The special task to present and discuss the experimental results train additional soft skills like the ability to communicate orally to individuals and groups and to tailor the oral communication to the level and experience of the audience. The students use appropriate scientific open source software (incl. R statistical computing language, Python general purpose programming language), graphics, and other tools to clarify complex information. The analysis of the data includes methods used in applied statistical bioinformatics and bioanalytical pharmacology.

Moreover, the accompanying seminar will train methodological competencies (e.g. media skills), and soft skills like presenting special facts and data and to communicate in a group.
Contents:
  • Sterile working technique, media preparation
  • Routine maintenance of cell lines: Medium replacement, subculture of adherent cells
  • Cryopreservation: Freezing and thawing of cells
  • Contamination control: Microscopical observation, etc.
  • Characterization of human (tumor) cells or cell lines
  • Morphology: microscopy of cells
  • Proliferation: Growth curve, population doubling-time
  • Testing of (mouse monoclonal) antibodies ((m)Ab). Immunocytochemistry  and immunochemistry with (m)Abs
  • Comparison of different cell assays e.g. gamma H2AX-Assays and FISH assays
  • Assesment of the effect of chemoterapeutic substances

Contents of the seminar:

Deepening the knowledge of the methods used in the labcourse. Methods in applied statistical bioinformatics, literate programming and report generation (Remarkdown or Jupyter notebook).

Recommended Prerequisites:Basic knowledge in Cell biology, Biochemistry, applied biostatistics and bioinformatics.
Mandatory Prerequisites:Only applies to the Master Biotechnology PO 2018 with 4 semesters standard period of study:
Successfull completion of module
  • 12739 - Research Internship
Forms of Teaching and Proportion:
  • Laboratory training / 7 Hours per Week per Semester
  • Seminar / 1 Hours per Week per Semester
  • Self organised studies / 120 Hours
Teaching Materials and Literature:
  • Freshney, Ian: Culture of Animal Cells: A Manual of Basic Technique and Specialized A pplications; 2010
  • Lindl, Toni.; Gstraunthaler G.: Zell- und Gewebekultur. Von den Grundlagen zur Laborbank; Spektrum Akademischer Verlag, 6. Auflage, 2008
  • Schmitz, Sabine: Der Experimentator Zellkultur, Spektrum Akademischer Verlag 2011
  • Logan, M., Biostatistical Design and Analysis Using R: A Practical Guide. 2010
  • Selvin, S., 1998. Modern Applied Biostatistical Methods: Using S-Plus, Monographs in Epidemiology and Biostatistics. Oxford University Press, Oxford, New York.
  • Lee, J.K., 2010. Statistical Bioinformatics: For Biomedical and Life Science Researchers. Wiley.
  • Sencar, H.T., Memon, N. (Eds.), 2013. Digital Image Forensics. Springer New York, New York, NY. https://doi.org/10.1007/978-1-4614-0757-7
  • National Research Council (US) Committee on A Framework for Developing a New Taxonomy of Disease, 2011. Toward Precision Medicine: Building a Knowledge Network for Biomedical Research and a New Taxonomy of Disease, The National Academies Collection: Reports funded by National Institutes of Health. National Academies Press (US), Washington (DC).
Module Examination:Continuous Assessment (MCA)
Assessment Mode for Module Examination:
  • Lab work-preparation of experiment, performance, discussion, time management (30%)
  • Presentation and discussion in the seminar, duration depends on the topic  (25%)
  • Presentation of results (25%)
  • Oral Test, duration: 15 min (20%)
Evaluation of Module Examination:Performance Verification – graded
Limited Number of Participants:8
Part of the Study Programme:
  • Master (applied) / Biotechnology / PO 2018
  • Master (applied) - Extended Semester / Biotechnology / PO 2018
Remarks:The module takes place as a 6 week block course.
Module Components:
  • Lab Course "Methods in Bioanalytics"
  • Seminar "Methods in Bioanalytics"
Components to be offered in the Current Semester: