12800 - Microbial Metabolism Modulübersicht

Module Number: 12800
Module Title:Microbial Metabolism
  Stoffwechsel der Mikroorganismen
Department: Faculty 2 - Environment and Natural Sciences
Responsible Staff Member:
  • Prof. Dr. rer. nat. habil. Stahmann, Klaus-Peter
Language of Teaching / Examination:English
Duration:1 semester
Frequency of Offer: Every winter semester
Credits: 5
Learning Outcome:Goal of the lecture is
  1. repetition of basics in bacterial and fungal metabolism and its regulation
  2. in depth understanding of selected topics based on recent original papers and current reviews
  3. to understand how conclusions are drawn from data, especially experimental data
  4. to understand how independent research strategies can lead to overlapping evidences
  5. to understand the difference qualitative and quantitative experimental approaches
  6. to understand the route starting from a scientific question over experimental data to a model explaining complex phenomena of microbiology
  7. to become able to work as a PhD student
Contents:Microbial Metabolism and Regulation
As a general principle all statements presented in the lectures are based on conclusions drawn from original data. Topics:
a) Mutations affecting metabolic pathways: (conditional) auxotrophy; supplementation; glycine biosynthesis; definded growth media;
b) Isoenzymes: isocitrate DHs; serine hydroxymethyltransferases; carbon flux analysis; subcellular compartmentation; aspartate kinases of E. coli
c) Regulation of metabolism: lac, ara, trp operon; repression, induction, attenuation, mRNA half-life control;; adenylation of glutamine synthetase; phosphorylation; feed back inhibition
d) Circadian clocks: control of photosynthesis and nitrogenase in Synechococcus and Anabaena; sporulation in Neurospora
e) Reporter systems: lacZ; green fluorescent protein; organelle targeting;
f) Quorum sensing: biofilms; signal systems in Gram-positive and Gram-negative bacteria;
Recommended Prerequisites:Basic knowledge in biochemistry, molecular biology, microbiology.
Mandatory Prerequisites:none
Forms of Teaching and Proportion:
  • Lecture / 2 Hours per Week per Semester
  • Self organised studies / 120 Hours
Teaching Materials and Literature:About 30 pdf-files of original research reports, review articles, PhD theses are available for the students e.g.
Biochem J 369: 263-73; PNAS 100: 5914-9; Genes Develop 15:1468-80; YEAST 21: 63-73; EMBO J 22: 2127-34; J Bacteriol 185: 2066-79;
Schlüpen C Diss Uni Düsseldorf; PNAS 90: 5672-6; PNAS 99: 9697-702; Microbiology 154: 2184-94; Mol Cell Biol 6221-8; BBA 1577: 240-50; J Mol Cat 10: 335-43; PNAS 98: 12221-6; J Bacteriol 182: 1-8; J Biol Chem 271: 11113-9; Microbiol 147: 3377-86; PNAS 107: 2043-47
Module Examination:Final Module Examination (MAP)
Assessment Mode for Module Examination:Written Examination (120 min)
Evaluation of Module Examination:Performance Verification – graded
Limited Number of Participants:None
Part of the Study Programme:
  • Abschluss im Ausland / Biotechnologie / keine PO
  • Master (applied) / Biotechnology / PO 2018
  • Master (applied) - Extended Semester / Biotechnology / PO 2018
Remarks:"Recommended Placement in the Study Plan"
Biotechnology (Master of Science, 3 Semester Version): Master 1st year course
Biotechnology (Master of Science, 4 Semester Version): Master 2nd year course
Module Components:
  • Lecture "Microbial Metabolism and Regulation"
  • Examination "Microbial Metabolism and Regulation"
Components to be offered in the Current Semester: