Chair of
Biochemistry
Prof. Dr. rer. nat. habil. Ralf Stohwasser
Research
Technologies/Equipment
Cell culture of mammalian cells (human and murine cell lines)
Cell cycle and apoptosis analyzed by flow cytometry (FACS Canto IITM, Becton Dickinson)
Cell lysis and subcellular fractionation
Ultracentrifugation of proteases complexes (proteasome, apoptosome; OptimaTM Max Ultracentrifuge, Beckman Coulter)
Cloning and mutagenesis technologies (construction of plasmids for overexpression of proteins in E. coli, SF21 insect cells; stable transfection of mammalian cells for gene overexpression or silencing; genome editing using CRISPR/Cas9)
Protein purification of recombinantly expressed or native proteins (ÄKTA primeTM & ÄKTA fplcTM; GE Healthcare)
ELISA and Multiparametric Bead Technologies (Cooperation: Innoprofile Groups at BTU)
Gel electrophoretic protein characterization (BNGE; NEPHGE, IEF; PHASTTM-System)
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