Technologies/Equipment

Research

Technologies/Equipment

Cell culture of mammalian cells (human and murine cell lines)
Cell cycle and apoptosis analyzed by flow cytometry (FACS Canto II^TM, Becton Dickinson)
Cell lysis and subcellular fractionation
Ultracentrifugation of proteases complexes (proteasome, apoptosome; Optima^TM Max Ultracentrifuge, Beckman Coulter)
Cloning and mutagenesis technologies (construction of plasmids for overexpression of proteins in E. coli, SF21 insect cells; stable transfection of mammalian cells for gene overexpression or silencing; genome editing using CRISPR/Cas9)
Protein purification of recombinantly expressed or native proteins (ÄKTA prime^TM & ÄKTA fplc^TM; GE Healthcare)
Enzymatics (Alkoholdehydrogenase, Proteasomes, Caspases, Peptidylarginine Deiminases)
ELISA and Multiparametric Bead Technologies (Cooperation: Innoprofile Groups at BTU)
Gel electrophoretic protein characterization (BNGE; NEPHGE, IEF; PHAST^TM-System)

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