Research
Technologies/Equipment
- Cell culture of mammalian cells (human and murine cell lines)
- Cell cycle and apoptosis analyzed by flow cytometry (FACS Canto IITM, Becton Dickinson)
- Cell lysis and subcellular fractionation
- Ultracentrifugation of proteases complexes (proteasome, apoptosome; OptimaTM Max Ultracentrifuge, Beckman Coulter)
- Cloning and mutagenesis technologies (construction of plasmids for overexpression of proteins in E. coli, SF21 insect cells; stable transfection of mammalian cells for gene overexpression or silencing; genome editing using CRISPR/Cas9)
- Protein purification of recombinantly expressed or native proteins (ÄKTA primeTM & ÄKTA fplcTM; GE Healthcare)
- Enzymatics (Alkoholdehydrogenase, Proteasomes, Caspases, Peptidylarginine Deiminases)
- ELISA and Multiparametric Bead Technologies (Cooperation: Innoprofile Groups at BTU)
- Gel electrophoretic protein characterization (BNGE; NEPHGE, IEF; PHASTTM-System)