11858 - Proteostasis Modulübersicht
| Module Number: | 11858 |
| Module Title: | Proteostasis |
| Proteostase | |
| Department: | Faculty 2 - Environment and Natural Sciences |
| Responsible Staff Member: |
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| Language of Teaching / Examination: | English |
| Duration: | 1 semester |
| Frequency of Offer: | Every summer semester |
| Credits: | 5 |
| Learning Outcome: | Proteostasis is a concept to summarize the contributions of protein synthesis, folding, modification, trafficking and degradation to steady state maintenance and dynamics of proteomes in cells. Major aim of the module is to deepen mechanistic knowledge in protein structure and function and the contributions of aforementioned processes during regulation of cellular homeostasis. The topic requires confrontation with complexity of living systems and scientific terminology. The consideration of mechanistic details of cellular processes should deepen the comprehension of important principles as foundations of cellular homeostasis and molecular pathology. Based on a mechanistic understanding of cell biological and molecular processes within the concept of protostasis, students are invited to contribute with their future work to progress in the understanding of the molecular pathology, diagnostics and therapy of human diseases (e.g. neurodegeneration, cancer, aging). Apart from these specific topics one major idea of the lecture is to enlighten the philosophy behind the scientific method. Historical milestones during the discovery of proteolytic systems involved in cell cycle control, apoptosis and cell survival signalling pathways, illustrated with original papers and reviews exemplifies deductive strategies and causality within scientific philosophy. In addition to specialized knowledge (professional competences), the students will train social competences because of being stimulated for contributions and critical discussions. |
| Contents: | Central topic of the module are cellular and molecular processes shaping the functionality of cellular proteomes. Protein synthesis, protein folding and misfolding, protein modification, subcompartimental localization and degradation are interconnected topics central to the understanding of molecular cell biology. The unique feature of this module is to look at these processes from the perspective of distinct proteolytic systems such as ubiquitin-dependent and independent proteasomal proteases, lysosomal proteases in autophagy, or caspases in apoptosis and inflammation and generally protease in antigen processing. Firstly, basics in protein biochemistry as well as technologies to analyze steady state and turnover of proteins are discussed. Next, the enzymology of proteases (protease families and mechanisms, inhibitors, diseases and therapy), proteolytic systems and protein modifications is a specific focus of this module. While considering complex proteolytic systems such as those of autophagy, apoptosis and the ubiquitin proteasome system, the module connects the topic of proteostasis specifically with cell survival signaling as exemplified by NFkB or p53 pathways, cell cycle regulation or apoptotic/inflammatory pathways. While emphasizing the role of proteases for cellular homeostasis, peptide generation as a principle of antigen processing should not be neglected. Hence, the discussion of MHC class I and class II pathways demonstrates the roles of proteolytic systems for the immune response. |
| Recommended Prerequisites: | Basic level in biochemistry, cell biology and molecular biology |
| Mandatory Prerequisites: | none |
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| Teaching Materials and Literature: | As a starting point textbooks of molecular cell biology and protein biochemistry are recommended:
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| Module Examination: | Final Module Examination (MAP) |
| Assessment Mode for Module Examination: | Written Examination (120 min) |
| Evaluation of Module Examination: | Performance Verification – graded |
| Limited Number of Participants: | None |
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| Remarks: | none |
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| Components to be offered in the Current Semester: |