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Module Number:
| 14122
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| Module Title: | Biomedical Lab Course III - Cell culture |
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Biomedizinischer Laborkurs III - Zellkultur
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| Department: |
Faculty 2 - Environment and Natural Sciences
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| Responsible Staff Member: | -
Dr. rer. nat. habil. Rödiger, Stefan
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| Language of Teaching / Examination: | English |
| Duration: | 1 semester |
| Frequency of Offer: |
Every winter semester
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| Credits: |
6
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| Learning Outcome: | - After completing the module, the students will be able to present the current literature on a research area in a summarized form. By presenting and discussing their results from the laboratory course they are able to critically question research results. In the practical course, students have to work on sophisticated projects which need competences such as teamwork, organized project planning (project management) and analytical abilities
- Acquire theoretical knowledge and practical experience in basic principles of cell culture techniques.
- Get familiar with the morpholgy, physiology and way to work with different animal and human cell lines.
- Generate awareness for the use of cells in culture (advantages and limitations).
- Understand the principles as well as application of cell biological methods to characterise the main parameters of cell behaviour like proliferation, differentiation, and viability.
- Discriminate the special features of cells growing as a monolayer in a culture flask (2D-culture).
- Applying advanced techniques of cell biological methods to characterise the main parameters of cell behaviour like proliferation, viability, toxicity, and differentiation.
- Knowledge and experience in microscopical techniques (Phase contrast, brightfield, fluorescence)
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| Contents: | - Sterile working technique, media preparation
- Routine maintenance of cell lines: Medium replacement, subculture of adherend and suspension cells, cell counting, cryopreservation, contamination control (microscopical observation, mycoplasma screening)
- Cultivation of cells in monolayer (2D)
- Application of special test systems to monitor proliferation, viability, and toxicity in monolayer cells
- Analyses of morphology of cells in 2D
- Working with different microscopic techniques: Phase contrast microscopy (basis “life cell” analyses), brightfield microscopy, fluorescence microscopy
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| Recommended Prerequisites: | none |
| Mandatory Prerequisites: | "Biomedical Lab Course II - Functional Bioanalytics", 14121 |
| Forms of Teaching and Proportion: | -
Seminar
/ 1 Hours per Week per Semester
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Laboratory training
/ 3 Hours per Week per Semester
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Self organised studies
/ 120 Hours
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| Teaching Materials and Literature: | - "Cell Culture for Biologists: A Practical Approach" by D. Rickwood and B.D. Hames - This text offers practical guidance on cell culture techniques, with a focus on sterile working practices.
- further required material will be provided (script for the experiments)
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| Module Examination: | Prerequisite + Final Module Examination (MAP) |
| Assessment Mode for Module Examination: | Prerequisite:
- successful completion of the occupational safety instruction, submission of the report on the experiments carried out during the laboratory training (35-45 pages), ungraded
Final Module Examination:
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| Evaluation of Module Examination: | Performance Verification – graded |
| Limited Number of Participants: | None |
| Part of the Study Programme: | -
Bachelor (research-oriented) /
Life Science and International Health /
PO 2025
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| Remarks: | None |
| Module Components: | - Seminar / 1 Hours per Week per Semester
- Laboratory training / 3 Hours per Week per Semester
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| Components to be offered in the Current Semester: | |
