12771 - Cells and Tissues - Culture and Evaluation Modulübersicht

Module Number: 12771
Module Title:Cells and Tissues - Culture and Evaluation
  Zell- und Gewebekultur
Department: Faculty 2 - Environment and Natural Sciences
Responsible Staff Member:
  • Dr. rer. nat. Hansen, Barbara
Language of Teaching / Examination:English
Duration:1 semester
Frequency of Offer: Every summer semester
Credits: 8
Learning Outcome:
  • After completing the module, the students will be able to present the current literature on a research area in a summarized form. By presenting and discussing their results from the laboratory course they are able to critically question research results. In the practical course, students have to work on sophisticated projects which need competences such as teamwork, organized project planning (project management) and analytical abilities
  • Acquire theoretical knowledge and practical experience in basic principles of cell culture techniques.
  • Get familiar with the morpholgy, physiology and way to work with different animal and human cell lines.
  • Generate awareness for the use of cells in culture (advantages and limitations).
  • Understand the principles as well as application of cell biological methods to characterise the main parameters of cell behaviour like proliferation, differentiation, and viability.
  • Engineering artificial tissues (3D-culture) using cell lines. Discriminate the special features of cells cultured in 3D and cells growing as a monolayer in a culture flask (2D-culture).
  • Applying advanced techniques of cell biological methods to characterise the main parameters of cell behaviour like proliferation, viability, toxicity, and differentiation.
  • Advanced knowledge and experience in microscopical techniques (Phase contrast, brightfield, fluorescence)
Contents:
  • Sterile working technique, media preparation
  • Routine maintenance of cell lines: Medium replacement, subculture of adherend and suspension cells, cell counting, cryopreservation, contamination control (microscopical observation, mycoplasma screening)
  • Cultivation of cells in monolayer (2D) and engineering of in vitro tissues of different cell types (3D-cell culture systems; spheroids)
  • Application of special test systems to monitor proliferation, viability, and toxicity  in monolayer cells
  • Analyses of morphology  of cells in 2D- and 3D (preparation of cryosections of spheroids, histological  analyses)
  • Working with different microscopic techniques: Phase contrast microscopy (basis “life cell” analyses), brightfield microscopy, fluorescence microscopy
Recommended Prerequisites:Basic knowledge in cell culture.
Mandatory Prerequisites:Only applies to the Master Biotechnology PO 2018 with 4 semesters standard period of study:
Successfull completion of module
  • 12739 - Research Internship
Forms of Teaching and Proportion:
  • Laboratory training / 7 Hours per Week per Semester
  • Seminar / 1 Hours per Week per Semester
  • Self organised studies / 120 Hours
Teaching Materials and Literature:
  • Culture of Animal Cells: A Manual of Basic Technique, Freshney R. I., 6th edition, Wiley-Blackwell, 2010
  • Zell- und Gewebekultur. Von den Grundlagen zur Laborbank, Lindl T und Gstraunthaler G., Spektrum Akademischer Verlag, 6. Auflage, 2008
  • Der Experimentator: Zellkultur, Spektrum Akademischer Verlag, 3. Auflage 2011
  • Molecular Biology of The Cell, Alberts et al., 5th/6th edition, Garland Sciences, 2008/2015
  • Specific original scientific publications
  • Manuals (SOPs) for cell culture & cell analytics of the Lab of Prof. U. Anderer
Module Examination:Continuous Assessment (MCA)
Assessment Mode for Module Examination:
  • Lab work-preparation of experiment, performance, discussion, time management (40%)
  • Presenting & Discussing Results (20%)
  • Presentation and discussion in the seminar, duration depends on the topic (20%)
  • Oral Test; duration 15 min (20%)
Evaluation of Module Examination:Performance Verification – graded
Limited Number of Participants:8
Part of the Study Programme:
  • Master (applied) / Biotechnology / PO 2018
  • Master (applied) - Extended Semester / Biotechnology / PO 2018
Remarks:The module takes place as a 6 week block course.
Module Components:
  • Lab Course "Cells and Tissues – Culture and Evaluation"
  • Seminar "Cells and Tissues – Culture and Evaluation"
Components to be offered in the Current Semester: