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Module Number:
| 14333
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| Module Title: | Downstream Processing for Products of Industrial Microbiology |
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Aufarbeitung von Produkten der Industriellen Mikrobiologie
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| Department: |
Faculty 2 - Environment and Natural Sciences
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| Responsible Staff Member: | -
Prof. Dr. rer. nat. habil. Stahmann, Klaus-Peter
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| Language of Teaching / Examination: | English |
| Duration: | 1 semester |
| Frequency of Offer: |
Every winter semester
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| Credits: |
6
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| Learning Outcome: | After completing the module, students should have acquired the following skills:
- Lecture: familiarization with industrial applications of processing methods for microbial products to gain an overview; understanding the relationships between material properties and methods of enrichment; recognizing the advantages and disadvantages of different separation methods; understanding the causes of economic successes and failures
- Seminar: practicing presentation and scientific discussion, i.e. logical and factual argumentation using appropriate technical language, concentrating on the essentials and dealing fairly with discussion partners. Seminar: Practicing presentation and scientific discussion, i.e. logical, factual argumentation in appropriate technical language, concentration on the essentials and fair treatment of discussion partners; developing a critical attitude towards experiments, results and conclusions; independent development of important connections from review articles and original papers
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| Contents: | - Lecture:
- The lecture deals with the recovery and purification of biotechnical products to achieve the desired specification while maximizing yield and minimizing process costs. Often down-stream processing is the most expensive part of the stream.
- The focus is firstly on sedimentation of cells and solid particles as well as on alternatives like flocculation and flotation, filtration and centrifugation. Disintegration techniques are discussed. Product isolation by extraction or sorption is a further chapter. Furthermore precipitation and chromatography as well as membrane separation, e.g. reverse osmosis or ultrafiltration, are interesting topics. Electrophoresis and drying are closing the separate discussion of single methods. After-wards combined operations are explained. A special chapter is the refolding of proteins, which have to gain function, e.g. enzymatic activity, after, e.g. production as inclusion bodies.
- Several examples show economic solutions: commercial enzymes, recombinant proteins, polysaccharides, antibiotics, organic acids, ethanol, and biomass.
- Seminar:
- Self-study of original publications, reviews and patents; presentation of a table or a figure with measurement data or scientific models; critical discussion of the data; comparison of the methods presented with alternative methods; discussion of the strengths and weaknesses of selected models
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| Recommended Prerequisites: | Basic knowledge of physics, chemistry, organic chemistry, biochemistry, molecular biology, microbiology, cell biology |
| Mandatory Prerequisites: | None |
| Forms of Teaching and Proportion: | -
Lecture
/ 2 Hours per Week per Semester
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Seminar
/ 1 Hours per Week per Semester
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Self organised studies
/ 135 Hours
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| Teaching Materials and Literature: | Textbooks:
Wilson DB, Sahm H, Stahmann KP, Koffas M (2020) Industrial Microbiology, Wiley-VCH, Weinheim
Jagschies, G., Lindskog, E., Lacki, K., & Galliher, P. M. (Eds.). (2018). Biopharmaceutical processing: development, design, and implementation of manufacturing processes. Elsevier.
Selected reviews, e.g. Hohmann HP, Stahmann KP (2010) Comprehensive Natural Products II. Chemistry and Biology, 10, 115-139.
Jungbauer, A. (2013). Continuous downstream processing of biopharmaceuticals. Trends in biotechnology, 31(8), 479-492.
Nunes, D. D., Pillay, V. L., Van Rensburg, E., & Pott, R. W. M. (2024). Oleaginous microorganisms as a sustainable oil source with a focus on downstream processing and cost-lowering production strategies: A review. Bioresource Technology Reports, 101871.
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| Module Examination: | Final Module Examination (MAP) |
| Assessment Mode for Module Examination: | Written exam graded 120 min |
| Evaluation of Module Examination: | Performance Verification – graded |
| Limited Number of Participants: | None |
| Part of the Study Programme: | -
Master (research-oriented) /
Chemistry: Materials, Engineering and Sustainability /
PO 2025
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| Remarks: | None |
| Module Components: | |
| Components to be offered in the Current Semester: | |
